Team:Imperial College London/Drylab/M1/Protein production/Analysis/Detailed

From 2009.igem.org

Revision as of 15:38, 14 September 2009 by Nuri (Talk | contribs)

In the absence of IPTG

Equation 1: Equation describing the rate of transcription of LacI MRNA (MLacI):
II09 MOD1 M1.jpg

kmlacI is the transcription rate of MlacI (a measure of promoter strength) and dmlacI is the degradation rate. At steady state:

II09 M1 SS1.jpg



Equation 2: Equation describing the rate of translation of LacI protein (PlacI)

II09 MOD1 M1 E2.jpg At steady state,

II09 M1 SS2.jpg

When IPTG is introduced

Parameters and values

Mr. Gene   Geneart   Clontech   Giant Microbes