Team:Imperial College London/Wetlab/Protocols/Autoinduction

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Section Assay Overview and Aims
Promoter Characterisation Secondary Carbon Source Diauxie Growth
  • To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling
  • To determine the best secondary carbon source for optimal growth
Auto-Induction Glucose Time Delay * Characterise the tunable time duration it takes before GFP expression (M2 activation)










Glucose time delay

Aims

  • Characterise the tunable time duration it takes before GFP expression (M2 activation)

Assay

  • The cells will be grown until OD= 0.7.
  • The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
  • OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
  • The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
  • The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)

See Glucose time delay for details

Mr. Gene   Geneart   Clontech   Giant Microbes

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