Team:Chiba/Notebook/Calendar/9 September 2009

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Check of Sender's AHL generation (1)

08:00 main culture

divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
WASH 3 times
Add 12.5ml new culture
Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
Do same operation every 15min to 2nd~10th tubes
after all, mix the supernatant and solid media at 1:1
make 10 concentration varieties of solid media

Lab Notebook

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