Imperial College London/Notebook/2 October 2009

Testing

OD calibration 4th run 2nd Oct

Protocol considerations

• Cells were grown overnight in 10ml of M9 media
• The cells were allowed to grow until a high OD in the late morning, then placed in cold room
• OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10^-4 to 10^-6
• 100ul of cells were inoculated into each plate
• The OD of the overnight cells were measured again after 3 hours on ice to check if cell number changes during dilution process

Results

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Conclusions

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Suggestions/Improvements

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