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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

Plasmid extraction

(Estimated time: 1 hour)

Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]

5 ml or 9 ml (for higher yields) overnight mini-culture QIAGEN QIAprep Spin Miniprep Kit

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