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Team:Kyoto/GSDD/Results
From 2009.igem.org
Reslts & Discussion
Discussion Before the Experiment
The difference between our designed device and YAC
In the case of YAC, both ends is teromeric site so when some end sequences of them cut off by the end replication problem, then the cut sequences will repaired and teromere keep its sequence. But in the case of our parts, the cut sequence will not repaired and the length will become shorter as cell division repeated. But the most problem is the cut length is different with each cell. We assumed the cut length of yeast in each one cell division might be about 100-200 base pair, this length is same with the average primer sequence in the ragging chain replication of yeast cells.
- 環状化の問題とチェックポイントの問題（要はDNA修復機構の問題）
- 個体のバラツキとその影響のかんして
