Team:Wisconsin-Madison/Restriction Digestion

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Restriction Enzyme Mapping

Use smallest micro cuvettes and mix (total volume 18 μL):

1.8 μL Buffer (10x)

0.18 μL BSA +3 (100x)

12 μL MiliQ H20

3 μL DNA

1 μL Enzyme (100x)

Incubate in water bath at 37 C for 2 hours:

3.6 μL Dye (6x)


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