Team:NCTU Formosa/Notebook/Calendar

It is the beginning today!!!!!!!!

Lesson：Parts, Devices and Biobricks.

Present 2008 IGEM project

Present everyone’s primary idea

To discuss everyone’s idea.

To collate everyone’s advantage.

To decide our final project.

To revise our project.

To design experiment.

Meeting

Meeting

Resuspend plasmid that all we need from plate

transform, to spread on the plate, then incubate for 20hr

Trans E. coli to new LB broth , incubate for 16hr

Extract plasmid and run gel to check

To digest the first part and the second part of the biobrick, ligation, transform and running agarose gel to check that if digestion is OK or not, then spread on the plate, then incubate for 20hr

Trans E. coli to new LB broth, incubate for 16hr

Extract plasmid.

To digest the third part and the fourth part of the biobrick, ligation, transform and runing agarose gel to check that if digestion is OK or not then spread on the plate, then incubate for 20hr

Trans E. coli to new LB broth incubate for 16hr

Extract plasmid

To digest the front part (8/23) and the behind part (8/26) of the biobrick, ligate, transform and run gel to check that if digestion is OK or not then spread on the plate, then incubate for 20hr

Trans E. coli to new LB broth, incubate for 16hr

Extract plasmid

Use another enzyme to digest to check if the plasmid is OK or not

Today we have a meeting that ...

f the plasmid is wrong then we repeat the digest, ligate, and transform again

科科的實驗

We have a meeting

To send all the plasmid to be sequenced

To send all the plasmid to be sequenced

To discuss our final result

We have a meeting