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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

Materials needed:

• iGEM 2009 plates
• PC with internet connection :-)
• ddH20
• 0.5 ml sterile Eppendorf tubes

• Find the correct position of the desired DNA from the iGEM 2009 plates
• Resuspend with ddH20 and transfer in an Eppendorf tubes
• Store at -20°C

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