Team:Wisconsin-Madison/Cloning Protocols

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Overview of Cloning Protocol Insert and Vector Solid Culture Liquid Culture Plasmid Isolation Restriction Enzyme Mapping for Verification Insert Only PCR to add BioBrick Restriction Cut Sites (Primer Design) Insert and Vector PCR Plasmid Cleanup Concentration Verification Digestion Digestion Clean Up Concentration Verification Ligation Transformation Colony PCR Screening Procedures for these can be found in Protocol Section Back to Protocols