Parts
Measurement Kit
Name | Part type | Registry link
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pSMB_MEASURE: Promoter Measurement plasmid (mammalian) | Plasmid backbone | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
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pSMB_REFERENCE | Plasmid | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]
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Core promoters
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.
Name | Part type | Registry link
|
JeT | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
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JeT proximal-CMV core | Regulatory | [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]
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Promoters created by RA-PCR
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
Name | Part type | Registry link
|
Constitutive promoter; 0.4 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
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Constitutive promoter; 1 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
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Constitutive promoter; 2 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
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Constitutive promoter; 2.5 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
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NF-κB regulated promoter | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
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pPARγ regulated promoter 1 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
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pPARγ regulated promoter 2 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]
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HEARTBEAT Predicted promoters
<--
Name | Part type | Registry link
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SREBP-Responsive, HB1 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203123 Part:BBa_K203123]
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SREBP-Responsive, HB3 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203127 Part:BBa_K203127]
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SREBP-Responsive, HB7 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203130 Part:BBa_K203130]
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SREBP-Responsive, HB8 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125]
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SREBP-Responsive, HB9 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203126 Part:BBa_K203126]
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SREBP-Responsive, HB11 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203135 Part:BBa_K203135]
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-->
coming soon...
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