Team:Kyoto/CiC/Experiment

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Experiment

Construction

HIV-TAT::(LALAAAA)3 expressing vector †RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer. (Forward primer;

cggaattcgcggccgcttctagagaaagaggagaaatactagATGTATGGACGTAAAAAACGTCGTGGACGTCGTCGTGGCGGCGGTCATCATCATCATCACCATGGCGG Reverse primer;

ctgcagcggccgctactagtaTTACGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGGCCACCGCCATGGTGATGATGATG)

Signal for TIM23 complex::GFP expressing vector †RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR.

First-stage PCR Forward primer;

TTTAAACCGGCGACCCGTACCCTGTGCTCTTCTCGTTATCTGCTGcgtaaaggagaagaacttttcactggagttg

Reverse primer;

agtgagctgataccgctcgc

Second-stage PCR Forward primer;

cggaattcgcggccgcttctagagaaagaggagaaatactagATGCTGAGCCTGCGTCAGTCTATTCGTTTTTTTAAACCGGCGACCCGTAC

Reverse primer;

agtgagctgataccgctcgc