Team:Uppsala-Sweden/Modelling

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Modelling of ethanol production

For our modell we have some basic assumptions that are

1. Co-evolution of the of the ethanol producing pathway, making km and kcat being highly similar for pdc as well as ADH


2. Assuming quasi steady state (Michaelis Menten Chinetics 

  e0=e+es



  Ek 1 2.png


Having the Km and Kcat values gives e0 from level of product formation. 

  Ek 3.png


Having e0 and letting kt go towards zero, (kt here represents the rate at which our substrate pyruvate "disappears" into the Krebbs cycle), gives ks 

  Ek 4.png


Assuming Ks to be constant within the interval and assuming steady-state 

  Ek 5.png


S is a function of f(x,y) where x is proportional to e0 and y is proportional to kt 

  Ek 6.png


Measure for different x and y and fit the function 

  Ek 6.png





Bioneer Biolegio Clontech Uppsala Genome Center

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