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Team:USTC/Standard & Protocol

Contents 1 Team:USTC/Standard & Protocol 1.1 Standard protocol 1.1.1 Minipreps 1.1.2 Digestion 1.1.3 Gel Extraction 1.1.4 Ligation 1.1.5 Transformation 1.1.6 Colony PCR

Standard protocol

Minipreps

Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414

Digestion

The digestion enzymes we use are listed:

Pst I Fermentas ER0611 3000U

EcoR I Fermentas ER0271 5000U

Spe I Fermentas ER1251 1500U

Xba I Fermentas ER0681 400U

Gel Extraction

Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354

Ligation

Transformation

Colony PCR

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