Team:USTC/Standard & Protocol

From 2009.igem.org

Revision as of 16:48, 20 October 2009 by Lch2009 (Talk | contribs)

USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Standard & Protocol

Contents

Standard protocol

Minipreps

Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414

Digestion

The digestion enzymes we use are listed:

Pst I Fermentas ER0611 3000U

EcoR I Fermentas ER0271 5000U

Spe I Fermentas ER1251 1500U

Xba I Fermentas ER0681 400U

Gel Extraction

Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354

Ligation

Transformation

Colony PCR