Experiment Rationale

To investigate the behaviour of the lamda-cI thermoinducible construct and show that when temperature is low (at 28 degrees Celsius), there is low fluorescence output. This shows that the genome deletion module is repressed. When temperature is raised to 42 degrees Celsius, fluorescence increases, indicating that the repression is lifted. We are looking at both absorbance and fluorescence data.

Absorbance data

Understanding of the fluorescence data requires normalization with cell growth data, in the form of optical density (absorbance).

28 degrees Celsius

Figure 1: Absorbance at 28 degrees Celsius

42 degrees Celsius