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Team:Calgary/Lab/Signalling
From 2009.igem.org
UNIVERSITY OF CALGARY
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LAB INDEX
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SIGNALLING CIRCUIT
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Figure 1. AI-2 signalling cascade in the absence (left) and presence (right) of AI-2. In the absence of AI-2, LuxQ autophosphorylates and subsequently phosphorylates the cytoplasmic protein LuxU, which passes its phosphate to LuxO. Phospho-LuxO complexes with transcription factor σ54 to activate the transcription of genes downstream of one of the five qrr4 promoters. The promoter depicted here is qrr4, as it is the one engineered into our system. (b) In the presence of AI-2, LuxQ changes from a kinase to a phosphotase, and thus removes the phosphate of LuxU, which subsequently removes the phosphate of LuxO. Nothing binds to the qrr4 promoter and therefore there is no expression of downstream genes.
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