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Team:BCCS-Bristol/Safety
From 2009.igem.org
BCCS-Bristol
iGEM 2009
iGEM 2009
Safety Issues
Precautions taken to ensure health and safety to researchers and to the public
As the idea of communication between a certain population (in this case E.coli) could raise issues in health and safety of the general public the following precautions were taken during the implementation of the project in the laboratory:
- Novel proteins handled (FhuA,OsmE,Fiu) were derived from DNA by gene cloning with PCR from E.coli MG1655 a laboratory strain with no toxic implications.
- Novel proteins handled where screened from the literature to ensure that they will have no toxicity effects.
- Experiments were implemented in a Level 1 Laboratory with access only by trained individuals.
- Students involved in experimental work in the laboratory were trained to an appropriate level to apply relevant techniques and use relevant equipment and where suitable were supervised whilst carrying out laboratory work.
- Laboratory workers were always clothed in appropriate manner (lab coat, gloves, safety spectacles).
- Laboratory workers sterilised their hands before and after laboratory work and before entering and exiting the lab at all times.
- No bacterial cultures exited the laboratory unless they were suitably packaged and accompanied by one of the team members whilst in transport and this only occurred where it was necessary to transport cultures from one laboratory to another.
- No purified DNA or biological material was left unattended at any time, and all DNA and biological material was suitably stored according to Level 1 Laboratory rules.
The BCCS-Bristol iGEM'09 team declares that to the best of its knowledge that none of the Biobricks created and submitted to the partsregistry database is toxic/lethal or in any way malicious to individuals, at the time of writing this (21 October 2009).
Biobricks created and entered to the partsregistry database can be found here.
