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From 2009.igem.org

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Team:USTC/Parts

Contents 1 Team:USTC/Parts 1.1 1 1.2 2 1.3 3 1.4 4 1.5 5 1.6 6 1.7 7 1.8 8 1.9 9 1.10 10 1.11 11 1.12 12 1.13 13 1.14 14 1.15 15 1.16 16 1.17 17 1.18 18 1.19 19 1.20 20

To get the information of Parts, pleast visit [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=USTC USTC 2009 Parts]

This year we have submitted 171 biobrick, some of which were well characterized by a lot of measurements and analysised by modeling.

Here we seperated all the parts into different sets to describe according to our progress.

Assembly Road Map_1: the rudimentary map.

Assembly Road Map_2: the advanced map added the variation function.

1

'	?	Name	Type	Description	Designer	Length
Sent	Works		Regulatory	pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS	Danqian Liu, Chao Li, Hao Jiang	72

2

'	?	Name	Type	Description	Designer	Length
Sent	Works		Reporter	pLux/Tet(K176000)(LuxR+,TetR-)->RBS+GFP+T	Chao Li,Danqian Liu,Hao Jiang	955

3

'	?	Name	Type	Description	Designer	Length
Sent	Works		Signalling	TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Chao Li, Hao Jiang	1059
Sent	Works		Signalling	TetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1059
Sent	Works		Signalling	TetR repressible AHL->PoPS Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1059
Sent	Works		Signalling	TetR repressible AHL->PoPS Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1059

4

'	?	Name	Type	Description	Designer	Length
Unsent	Issues		Translational_Unit	PoPS->RBS+ccdB->PoPS	Zongxiao He, Hao Jiang	324
Sent	Issues		Translational_Unit	PoPS->RBS+lacZalpha-ccdB->PoPS	Zongxiao He, Hao Jiang	498
Sent	Issues		Generator	PoPS->RBS+ccdB+T	Zongxiao He, Hao Jiang	461
Sent	Issues		Generator	PoPS->RBS+lacZalpha-ccdB+T	Zongxiao He, Hao Jiang	635
Sent	Issues		Translational_Unit	PoPS->RBS(weak)+ccdB->PoPS	Zongxiao He, Hao Jiang	326
Sent	Issues		Translational_Unit	PoPS->RBS(weak)+lacZalpha-ccdB->PoPS	Zongxiao He, Hao Jiang	500
Sent	Issues		Generator	PoPS->RBS(weak)+ccdB+T	Zongxiao He, Hao Jiang	463
Sent	Issues		Generator	PoPS->RBS(weak)+lacZalpha-ccdB+T	Zongxiao He, Hao Jiang	637
Sent	Issues		Coding	ccdB-LVA coding sequence	Danqian Liu, Zongxiao He, Hao Jiang	342
Unsent	Issues		Coding	lacZalpha-ccdB-LVA coding sequence	Danqian Liu, Zongxiao He, Hao Jiang	513
Sent	Issues		Translational_Unit	PoPS->RBS+ccdB-LVA->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	360
Sent	Issues		Translational_Unit	PoPS->RBS+lacZalpha-ccdB-LVA->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	531
Unsent	Issues		Translational_Unit	PoPS->RBS(weak)+ccdB-LVA->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	362
Sent	Issues		Translational_Unit	PoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	533
Sent	Issues		Generator	PoPS->RBS+ccdB-LVA+T	Danqian Liu, Zongxiao He, Hao Jiang	497
Sent	Issues		Generator	PoPS->RBS+lacZalpha-ccdB-LVA+T	Danqian Liu, Zongxiao He, Hao Jiang	668
Unsent	Issues		Generator	PoPS->RBS(weak)+ccdB-LVA+T	Danqian Liu, Zongxiao He, Hao Jiang	499
Sent	Issues		Generator	PoPS->RBS(weak)+lacZalpha-ccdB-LVA+T	Danqian Liu, Zongxiao He, Hao Jiang	670

5

'	?	Name	Type	Description	Designer	Length
Sent	Works		Regulatory	constitutive promoter family member J23115 actual sequence (pCon 0.15)	Hao Jiang, Danqian Liu, Chao Li	35
Sent	Works		Regulatory	constitutive promoter family member J23107 actual sequence (pCon 0.36)	Hao Jiang, Danqian Liu, Chao Li	35

6

'	?	Name	Type	Description	Designer	Length
Sent	Works		Reporter	pCon max(J23119)->RBS+GFP+T	Chao Li,Danqian Liu,Hao Jiang	918
Sent	Works		Reporter	pCon 1.00(J23100)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCon 0.70(J23101)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCon 0.36(K176009)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCon 0.15(K176008)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCon 0.04(J23109)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCon 0.01(J23103)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	918
Sent	Works		Reporter	pCI(R0051)(lambda CI-)->RBS+GFP+T	Chao Li, Danqian Liu, Hao Jiang	932

7

'	?	Name	Type	Description	Designer	Length
Unsent	Fails		Generator	pCon max(J23119)->RBS+luxR+T	Chao Li,Danqian Liu,Hao Jiang	979
Sent	Works		Generator	pCon 0.70(J23101)->RBS+luxR+T	Danqian Liu, Chao Li, Hao Jiang	979

8

'	?	Name	Type	Description	Designer	Length
Sent	None		Signalling	pCon max(J23119)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	None		Signalling	pCon 1.00(J23100)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	Works		Signalling	pCon 0.70(J23101)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	Works		Signalling	pCon 0.36(K176009)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	Works		Signalling	pCon 0.15(K176008)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	Works		Signalling	pCon 0.04(J23109)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841
Sent	Works		Signalling	pCon 0.01(J23103)->RBS+luxI-LVA+T	Danqian Liu, Chao Li, Hao Jiang	841

9

'	?	Name	Type	Description	Designer	Length
Sent	Works		Signalling	TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Chao Li, Hao Jiang	1942
Sent	Works		Signalling	TetR repressible AHL->GFP Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	1942
Sent	Works		Signalling	TetR repressible AHL->GFP Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	1942
Sent	Works		Signalling	TetR repressible AHL->GFP Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	1942

10

'	?	Name	Type	Description	Designer	Length
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB	Zongxiao He, Hao Jiang	1528
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB	Zongxiao He, Hao Jiang	1702
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Zongxiao He, Hao Jiang	1530
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Zongxiao He, Hao Jiang	1704
Unsent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1530
Unsent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1704
Unsent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	1564
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	1735
Unsent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1566
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1737
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1530
Unsent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1704
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1530
Sent	Issues		Signalling	TetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	1704

11

'	?	Name	Type	Description	Designer	Length
Sent	Works		Generator	PoPS->RBS+tetR(no LVA)+T	Chao Li,Danqian Liu,Hao Jiang	782
Sent	Works		Generator	PoPS->RBS+tetR-LVA+T	Chao Li,Danqian Liu,Hao Jiang	840

12

'	?	Name	Type	Description	Designer	Length
Sent	Works		Device	Repressible AHL->GFP Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->GFP	Chao Li, Danqian Liu, Hao Jiang	2732
Sent	Works		Device	Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Chao Li, Danqian Liu, Hao Jiang	2790
Sent	Works		Device	Repressible AHL->PoPS Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->PoPS	Chao Li, Danqian Liu, Hao Jiang	1849
Sent	Works		Device	Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Chao Li, Danqian Liu, Hao Jiang	1907
Unsent	Fails		Device	Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1907
Sent	Works		Device	Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2790
Unsent	Fails		Device	Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1907
Unsent	Fails		Device	Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2790
Unsent	Fails		Device	Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1907
Unsent	Fails		Device	Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2790

13

'	?	Name	Type	Description	Designer	Length
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1892
Sent	None		Device	aTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1950
Sent	None		Device	aTc&AHL->GFP: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2775
Sent	None		Device	aTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.36->tetR+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1892
Unsent	Works		Device	aTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1950
Sent	None		Device	aTc&AHL->GFP: pCon 0.36->tetR+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2775
Sent	Works		Device	aTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.15->tetR+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1892
Unsent	Works		Device	aTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1950
Sent	None		Device	aTc&AHL->GFP: pCon 0.15->tetR+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2775
Sent	Works		Device	aTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2833
Unsent	Works		Device	aTc&AHL->PoPS: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1892
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Zongxiao He, Hao Jiang	1950
Sent	Works		Device	aTc&AHL->GFP: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2775
Unsent	None		Device	aTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Sent	Works		Device	aTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Sent	None		Device	aTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Sent	Works		Device	aTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.04->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Sent	None		Device	aTc&AHL->GFP: pCon 0.04->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Sent	Works		Device	aTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Unsent	None		Device	aTc&AHL->GFP: pCon 0.70->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.36->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Unsent	None		Device	aTc&AHL->GFP: pCon 0.36->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.15->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Unsent	None		Device	aTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.04->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Unsent	None		Device	aTc&AHL->GFP: pCon 0.04->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833
Unsent	None		Device	aTc&AHL->PoPS: pCon 0.01->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1950
Unsent	None		Device	aTc&AHL->GFP: pCon 0.01->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2833

14

'	?	Name	Type	Description	Designer	Length
Unsent	Works		Device	PoPS*cell density->PoPS: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1865
Sent	Works		Device	cell density->PoPS: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Sent	Works		Device	cell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS	Danqian Liu, Hao Jiang	1908
Unsent	Issues		Device	Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2510
Sent	Issues		Device	Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Unsent	Issues		Device	Cell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Sent	Issues		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Sent	Issues		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Sent	Issues		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Sent	Issues		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Sent	Issues		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Hao Jiang	2553
Unsent	Issues		Device	Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2336
Sent	Issues		Device	Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Sent	Issues		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Sent	Issues		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Sent	Issues		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	cell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1908
Unsent	Issues		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2553
Unsent	Issues		Device	cell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1908
Unsent	Issues		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2553
Unsent	Issues		Device	cell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1908
Unsent	Issues		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
Unsent	Issues		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2553
	Fails		Device	cell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS	Danqian Liu, Zongxiao He, Hao Jiang	1908
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2379
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2553
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2413
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2584
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2415
	Fails		Device	Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2586
	Fails		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2413
	Fails		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2584
	Fails		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2415
	Fails		Device	Cell density controller: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2586
	Fails		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2413
	Fails		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2584
	Fails		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2415
	Fails		Device	Cell density controller: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2586
	Fails		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2413
	Fails		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2584
	Fails		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2415
	Fails		Device	Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2586
	Fails		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2413
	Fails		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA	Danqian Liu, Zongxiao He, Hao Jiang	2584
	Fails		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2415
	Fails		Device	Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)	Danqian Liu, Zongxiao He, Hao Jiang	2586
Sent	Works		Device	cell density->GFP: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2791
Sent	Works		Device	cell density->GFP: pCon 0.36->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2791
Sent	Works		Device	cell density->GFP: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2791
Sent	Works		Device	cell density->GFP: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2791
Sent	Works		Device	cell density->GFP: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP	Danqian Liu, Zongxiao He, Hao Jiang	2791

15

'	?	Name	Type	Description	Designer	Length
Sent	Works		DNA	Reverse primer VR3 (K176047) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR4 (K176048) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR5 (K176049) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR6 (K176050) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR7 (K176051) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR8 (K176052) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR9 (K176053) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR10 (K176054) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR11 (K176055) binding site	Jiayi Dou, Hao Jiang	20
Sent	Works		DNA	Reverse primer VR12 (K176056) binding site	Jiayi Dou, Hao Jiang	20

16

'	?	Name	Type	Description	Designer	Length
Sent	Works		Regulatory	constitutive promoter family member J23119 (pCon max) with primer VR10 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member J23100 (pCon 1.00) with primer VR3 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member J23101 (pCon 0.70) with primer VR4 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member K176009 (pCon 0.36) with primer VR7 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member K176008 (pCon 0.15) with primer VR9 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member J23109 (pCon 0.04) with primer VR8 site	Jiayi Dou, Hao Jiang	63
Sent	Works		Regulatory	constitutive promoter family member J23103 (pCon 0.01) with primer VR5 site	Jiayi Dou, Hao Jiang	63

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