Virginia Commonwealth/17 July 2009
From 2009.igem.org
Contents |
Friday 17 July 2009
Results
- Results of overnight culture was successful
- NEB 10 beta electrocompetency was successful
- NEB on LB had lawn growth
- NEB on LB after being shocked had lawn growth
- Electrophoresis test on J06702 and pSB4C5
- A DNA band was visible for both
Trentay 16:54, 17 July 2009 (UTC)
Craig and Clay
- There is still no growth on the kanamycin and chloramphenicol plates.
Tasks
Maria and Afton
- Religate promoters that did not grow in the first transformation (backbone pSB4C5)
- J23101 w/ E0240
- J23105 w/ E0240
- Run an electrophoresis test on J06702 and pSB4C5
Trentay 15:53, 17 July 2009 (UTC)
Wetlab
Maria and Afton
- Cryogenic stock of the following BioBricks was made (2 vials each)
- J23101 w/ J06702
- J23102 w/ E0240
- J23102 w/ J06702
- J23103 w/ E0240
- J23103 w/ J06702
- J23104 w/ E0240
- J23104 w/ J06702
- J23105 w/ J06702
- J23107 w/ E0240
- J23107 w/ J06702
- Electrophoresis test
Trentay 15:53, 17 July 2009 (UTC)