Template:Team:KULeuven/29 July 2009/BlueLightReceptor
From 2009.igem.org
GFP (
- miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
- a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:
6μl DNA 2μl buffer H 1μl EcoRI and 1μl XbaI 10μl AD
- the mixture was incubated for at least an hour at 37°C