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CAROL
Transformation of R0040 (promoter) +pCS26 (vector) into XL Gold cells
- From the overnight digest of R0040 with XhoI and BamHI, the promoter was ligated with vector, pCS26.
- These plasmids were transformed into XL Gold cells and was plated on Kanamycin plates overnight.
Team meeting:
- Lab:discussed about promoter library and the failure to see the expression of luciferase. Also, discussed about the primer design of R0040, so that we can clone the constitutively ON promoter in front of luciferase. This will show us what the luciferase expression will look like.
- Modelling: Discussed about Robustness. The three things that we decided to look at for robustness is: 1. PQ expression (the selection of the 'best' sigma 70 promoter) 2. Temperature Alterations (to see if changes in temperature (from 37oC to another temperature would affect expression) 3. Type of media (LB vs. LM)
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CHINMOYEE
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EMILY
Sequencing of J13002-LuxOD47E-B0015 Construct in psB1AC3
- Sent Colony 3 of my J13002-LuxOD47E-B0015 construct down for sequencing, results will come in on Monday hopefully.
- Explored Secondlife and met with Rob from UChicago.
- Prepared the Human Practices project write-up for the Wiki.
- Team Meeting- Talked about the exploration of seminars in Second Life and what ideas that has given us. Talked about the pending completion of the J13002-LuxOD47E-B0015 curcuit, results will come in on Monday.
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FAHD
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IMAN
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JAMIE
Verification of cl lambda inverter in psB2K3
- Plasmid miniprep of overnight cultures with Sigma Genelute Miniprep kit. Standard manufacturer protocol used; elution in 40µL of ddH2O.
- Vacufuge to concentrate.
- Verification digest with XbaI and PstI. Control: cl lambda part from the Registry distribution plate.
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JEREMY
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KATIE
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KEVIN
cPCR of the reporter circuit
In order to verify cPCR, I have once again ran a cPCR of the reporter circuit, Pqrr4+B0034(RBS)+K082003(GFP+LVA) with Pqrr4 Forward and Biobrick CP Reverse primers. Of course, pTaq was used to amplify the DNA. The product was then ran on 2% agarose gel at 90V. ----the gel is being run right now, will edit---
Distribution of our baking sale posters
Fahd left a stack of Baking sale posters for us to post throughout the building, and they were posted before 11am today.
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MANDY
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PATRICK
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PRIMA
Continuation of last day's work
Today, I edited the high school presentation proposition again and sent it out to Mrs. Ellechuk. I filled in the lab notebook and filled out the daily updates on the wiki.
I finished typing out the acknowledgement for each mentor/advisor of iGEM Calgary. I also helped to write references for Sonja. Then I finished off with mailing out more newsletters. We also had a very productive team meeting today.
On Monday I have to follow up with the companies which I have been contacting for the past few days. I will also call our hotel in Boston to change a few rooms around.
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STEFAN
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VICKI
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