Template:Team:KULeuven/3 August 2009/VanillinProduction

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We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation.

  • Sam5 + RBS in pSB1A2 (with PstI site removed)
  • Sam8 + RBS in pSB1A2
  • Sam8 in pSB1A2 coding sequence,
  • ComT in pSB1A2 coding sequence,

The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.

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