Uppsala-Sweden/4 August 2009

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Revision as of 12:32, 4 August 2009 by Flormane (Talk | contribs)




Ethanol Evolution

1,15ml of 95 EtOH were added to a 26ml flask from the recovery protocol of the ethanol evolution project. Thus resulting in approx. 4% EtOH concentration (0,03981% in theory) but I expect that some water evaporated during the culturing thus the former volume (26ml) was decreased. Flask were incubated in 30°C growth room on 120rpm shaker.

Butanol Evolution

26ml flasks from the Butanol recovery were supplied with isobutanol regarding their former concentration. (0,25% | 0,25% | 0,5% | control w/o). Flask were incubated in 30°C growth room on 120rpm shaker.

PCR (Kivd, ADH2 and PpetE) and Gel run

This is continued since yesterday.

We ran the PCR products on a gel with the following setup and result.

Setup: Media:gel_run_090804_adh2_kivd_ppete.xls

Result: 20090804 2 RT-PCR alr2938F-R no,33-40.jpg --Flormane 12:32, 4 August 2009 (UTC)

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