KEVIN
1. Plasmid Isolation of Pqrr4+B0034+K082003
Pqrr4+B0034+K082003 was isolated in order to verify its presence using Restriction digest and Sequencing later on. The product's purity and concentration were measured using Nanodrop utility and spectrophotometer.
{| border="1"
| '''Plasmid''' || '''260/280''' || '''260/230''' || '''Concentration [ng/μL]'''
|-
| ''Pqrr4''+B0034+K082003 C9 #1|| 2.02 || 2.72 || 158.0
|-
| ''Pqrr4''+B0034+K082003 C9 #2|| 2.06 || 3.61 || 160.4
|}
2. Restriction digest of Pqrr4+B0034+K082003
Restriction digest on Pqrr4+B0034+K082003 to verify its presence. This is our reporter circuit. The product was ran on 1.5% gel at 90V.
[[Image:Calgary_2009.08.05.Pqrr4%2BB0034%2BK082003.png|700px]]
3. Plasmid Switch of Pqrr4+I13500
Yesterday, I have done an overnight digestion of Pqrr4+I13500 and Q04510 (inverter) part in pSB2K3 vector. Today, with this product, I have performed phophatase treatment on Q04510 and ligated them together. This product was then transformed into TOP10 cells, and is now growing in the incubator at 37˚C.
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