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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Bake Sale day
This was my first bake sale.I made 3 cakes for sale: Marble and two choclate . The sale was a sucess . We raised more money than the last sale.
On the Modelling side :
We had a modelling meeting in the morning . Here we made a clear plan on what needs to be done . A tentative timeline was made . Work was designated . More planning was done for the presentation on Friday.
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EMILY
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FAHD
Marketing for August 5th (2009-08-05)
Today, I concentrated my energies on fundraising for our research project and finding potential media partners.
For the fundraising part, we conducted a bake sale from which, we raised $494.18 CDN Dollars. Every team member had contributed to the endeavour. I also contacted some airline agencies to get a quote for our trip to MIT, Boston.
I also contacted our University of Calgary Community Radio Station called CJSW 90.9 for doing a media coverage for our research project this year. I will do a follow-up later this week.
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IMAN
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JAMIE
Verification of Surette vector
- Ran digested Surette vector and undigested product on 0.7% agarose gel. No bands in digested product?
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JEREMY
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KATIE
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KEVIN
1. Plasmid Isolation of Pqrr4+B0034+K082003
Pqrr4+B0034+K082003 was isolated in order to verify its presence using Restriction digest and Sequencing later on. The product's purity and concentration were measured using Nanodrop utility and spectrophotometer.
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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| Pqrr4+B0034+K082003 C9 #1 | 2.02 | 2.72 | 158.0
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| Pqrr4+B0034+K082003 C9 #2 | 2.06 | 3.61 | 160.4
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2. Restriction digest of Pqrr4+B0034+K082003
Restriction digest on Pqrr4+B0034+K082003 to verify its presence. This is our reporter circuit. The product was ran on 1.5% gel at 90V.
The bands in Pqrr4+B0034+K082003 lanes seems to match the size I expected, which is around 1043bp; thus I can move forward with sequencing tomorrow.
3. Plasmid Switch of Pqrr4+I13500
Yesterday, I have done an overnight digestion of Pqrr4+I13500 and Q04510 (inverter) part in pSB2K3 vector. Today, with this product, I have performed phophatase treatment on Q04510 and ligated them together. This product was then transformed into TOP10 cells, and is now growing in the incubator at 37˚C.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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