LacI-Latch Notebook

Week 1: Research and Planning 08/06/09 - 12/06/09

Day 1 Monday (08/06/09)

• Researching the Registry for Biobricks
  • Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.

Day 2 Tuesday (09/06/09)

• Researching the Registry for Plasmids.
  • Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.

Day 3 Wednesday (10/06/09)

• Literature search for Parameters of T7 promoter and the Holin system.
  • Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
  • Identified the novagen plasmids pLysS and pLysE as possible solutions.

• Researching the Registry for LacI Repressor and T7 Polymerase
  • Identified K145014, K145001, I732100 for further discusion

Day 4 Thursday (11/06/09)

Day 5 Friday (12/06/09)

Week 2 15/06/09 - 19/06/09

Week 3 22/06/09 - 26/06/09

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09

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