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CAROL
Preparing Competent Cells
- Transformed plasmid, pCS26 into Top 10 cells with NEB Quick Ligase Buffer and Anarctic Phosphatase Buffer to verify if the E.coli cells are able to uptake the larger plasmid. We wanted to check the transformation efficiency of a 10KB plasmid. This was plated on LB+Kan plates.
- To prepare for making competent cells, we grew overnight cultures in 5 mL of LB broth of Top 10 cells and XL Gold Ultracompotent cells (Stratagene)
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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