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CAROL
Gel Extraction of luxCDABE
- The main purpose of gel extraction is to ensure that only luxCDABE is present for cloning. The problem with cloning luxCDABE is that we are cloning in smaller pieces of DNA, rather than the gene of interest.
- Prepared 0.7% gel and ran the gel for 90 minutes at 90V.
- Followed QIAquick Gel Extraction Kit Protocol (please see protocol page for details)
- The concentrations that we ended up getting from the gel extraction is low. We re-ran the 0.7% gel with a larger amount of product, however, the kit is only optimal for 3-4 kbs.
- Gradient PCR of luxCDABE with the biobrick sites at the end. (We ran out of PCR products to work with)
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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