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CAROL
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WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
Success
I was able to combine all three scripts into one by using the listen event and now it is possible for me to add more types of DNA to PCR and I can continue to easily add quite a few of them. I also was able to fix the error I was getting, which happened because as long as the first item stored in a list was found in the machine’s inventory, it believed everything was there, but when it went to do something with it, the item did not exist and an error occurred.
However, I would still like to change the way it is being used at this time since it is not really efficient to re-declare a variable every time you go through a loop. I will work on changing this at a later date.
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KEVIN
Plasmid Isolation of LuxCDABE (luciferase)
The LuxCDABE is being isolated by Caron Chan, but she needed some hands on this, and I was willing to help. LuxCDABE was isolated with Quigen's EndoFree Plasmid Maxi (quigen) according to the specification of the provider. A different kit than the one used in previous experiments was used because the LuxCDABE is very big in size (6kb), and the Minprep would not be capable of isolating the plasmid at a high enough concentration.
Despite of the use of Maxiprep, however, the spectrophotometer seemed to have not picked up any plasmid in the isolated sample, as the purity and concentration were below acceptable. Another Maxiprep may be needed to be done.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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