Team:KU Seoul/Notebook

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Lab

How do I test the transformation efficiency of my competent cells?
- Please see the protocol on how to [http://www.openwetware.org/wiki/TOP10_chemically_competent_cells make competent cells] for instructions on how to calculate transformation efficiency.

10.07.09 We had a review on our two ideas from Prof.Choi. He told us the ideas are creative and good but not fit for IGEM's concept. We need more feedback and information on our ideas. We need to get more familiar with IGEM's tools. Two additional ideas about Scromboid food poisoning and lactic acid degradation were referred at the end.

17.07.09 Till our next meeting, we should study how systemic biology works and understand related tools. Cumulate ideas. In other words, brainstorm consistently. Hyuk-jin will help with setting up experiments. Lab note opend.

24.07.09 We narrowed our ideas in to two. They are "Metal Detector" and "Bactaeria Compass." Think about what parts would be used and how you would build the circuits. Visit the former iGEM teams and study how they carried on their projects.

31.07.09 Find out the exact mechanism of metal detection. Look up the parts that are available on iGEM home. See how various metals can be detected by using E.coli.

07.08.09 We decided to detect 4 metals; Cd, Hg, Pb, and Ars. They are all critical to the health if ingested. Think about what the outcome would be after detection. Because there are 4 metals, you can bring out 16 different results by combining them. It seems that the most vialbe instrument would be light.

- Biosurfactants

- Keratinase

- Deodorant

- Perfume-producing bacteria

- Heat absorber

- Bio pencil

Should we focus on the result, or the procedure?

[http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (한글)]