Template:Team:KULeuven/2 September 2009/BlueLightReceptor

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Revision as of 14:47, 2 September 2009 by AnneV (Talk | contribs)
  1. FACS measurements:
    • the cultures from shift 2 had similar results as shift one. however, the GFP signal measured from the LigA construct was stronger. thus, cells definitely need to grow before undergoing irradiation.
  2. the dillutions and the shift one cultures put overnight did not show any significant results.
  3. was miniprepped and nanodropped
Part concentration (ng/μl) 260/280 λ
(A) 238,9 1,95
(B) 248,8 1,91
  1. after nanodropping, was restricted with SpeI and PstI, while was cut with PstI and XbaI.
  2. a gel electroforesis and extraction was performed. this showed a 800 bp signal at the lanes with , which is not expected.
  3. DB3.1, J23101 and LigA were ented in liquid culture and grown overnight to make glycerol stocks and competent cells
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