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CAROL
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WIKI CODING HERE
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CHINMOYEE
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EMILY
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FAHD
Bake Sale Sequel, June 30th 2009
Today, I helped organize our second bake sale (Cultural Bake Sale: The Sequel) of our marketing campaign. The bake sale managed to raise more than 370 dollars. We had cool baked items such as samosas, spring rolls, muffins and lots lots and lots of other stuff. I want to thank my entire iGEM calgary team for their tremndous support and countless effort the put in for the event.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Search for other antifreeze proteins
Carrot's antifreeze proteins were also looked into.
- 1099bp long
- They have a relatively weak TH activity of ~0.1-0.5°C, which is less than that of fish AFPs and significantly less than that of insect AFPs.
- The TH activity of recombinant proteins from an E.coli expression system is weak
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MANDY
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PATRICK
HUD Details
Retrospective Notebook: This entry was not written on this day, but derived later from working notes I made that day.
Spent today working out some details of how the tile control system would work. Should I erase the entire screen before drawing the next one, or just let the next screen's text and buttons replace all of the current display? Decided to blank first instead of relying on the next screen to replace it all. Sending a single message to all display tiles to pre-blank had a low overhead cost, the update process looked cleaner on the HUD, and I could then skip space characters when processing the next screen's text. (No need to tell tile x to display a space character, if it has been pre-blanked).
Set up a system for parsing lists of characters into lines on the UI, so that I could easily visualize what the display would look like in the program's code. e.g., an early version of the main menu:
display += " Biobrick ";
display += " Simulator ";
display += " ";
display += " X Introduction";
display += " X Levels ";
display += " X Sandbox ";
display += " X Help ";
display += " ";
The Xes here stand in for buttons.
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PRIMA
Marketing
Today, I followed up with almost all the companies which I was in charge of contacting and sent them the June Newsletter. I thanked all those companies who have sponsored our project and encourageed everyone to visit our wiki for details on our progress. I also attached the CTV link to every email.
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STEFAN
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VICKI
Sequencing of the LuxOD47A BBk + B0015 colonies
Based on yesterday's NotI restriction digest results, the LuxOD47A + B0015 colonies look promising. These were prepared with BBk construction primers and sent for sequencing, with positive results eventually confirmed. These are attached below.
Construction protocol: adding J13002 to LuxOD47A + B0015
Cutting with (EcoRI+SpeI for the J13002 part) and (EcoRI+XbaI for the LuxOD47A + B0015 part) restriction enzymes, Antarctic phosphatase treatment, ligation, transformation into competent TOP10 cells and plating on AK plates were all done in accordance with the procedures outlined on the protocol page. If this is done properly, the net J13002 + LuxOD47A + B0015 will be on an pSB1AK3 plasmid.
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