Biobricks

Coding Sequences

Mechanism

The first module involves engineering E. coli to synthesise the protein drug of interest to sufficient quantities. Once accomplished, activation of module two initiates the encapsulation phase, in which E. coli coats itself in a protective layer of colanic acid to form the E.ncapsulator. This protective capsule is what shields the protein drug against the harsh acidic environment of the stomach. The third module, genome deletion, is composed of a ‘suicide trigger’ mechanism that destroys the genetic material of the bacteria. Finally, once in the small intestine, the capsule will be naturally degraded by enzymes present, thereby releasing the designed biopharmaceutical to the gut where it can carry out its intended function.

RcsB: Colanic acid global regulator
Part BBa_K200000 : [http://partsregistry.org/Part:BBa_K200000 Link]

Dam: DNA Adenine Methylase
Part BBa_K200001 : [http://partsregistry.org/Part:BBa_K200001 Link]

ygiV (B3023): Colanic acid global regulator
Part BBa_K200002 : [http://partsregistry.org/Part:BBa_K200002 Link]

WaaL Ligase
Part BBa_K200003 : [http://partsregistry.org/Part:BBa_K200003 Link]

OtsA: Part 1 of 2 for trehalose producing enzymes
Part BBa_K200005 : [http://partsregistry.org/Part:BBa_K200005 Link]

OtsB: Part 2 of 2 coding for trehalose producing enzymes
Part BBa_K200006 : [http://partsregistry.org/Part:BBa_K200006 Link]

Cellulase
Part BBa_K200007 : [http://partsregistry.org/Part:BBa_K200007 Link]

PAH : Phenylalanine Hydroxylase
Part BBa_K200008 : [http://partsregistry.org/Part:BBa_K200008 Link]

Coding Sequence for the Restriction Enzyme DpnII
Part BBa_K200009 : [http://partsregistry.org/Part:BBa_K200009 Link]

Coding sequence for the Restriction Enzyme TaqI
Part BBa_K200010 : [http://partsregistry.org/Part:BBa_K200010 Link]

Temperature Sensitive lamda cI Repressor
Part BBa_K200011 : [http://partsregistry.org/Part:BBa_K200011 Link]

Lambda Promoter (cIts responsive)
Part BBa_K200012 : [http://partsregistry.org/Part:BBa_K200012 Link]