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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

Find the correct position of the desired DNA from the iGEM 2009 plates Resuspend with ddH20 and transfer in an Eppendorf tubes Store at -20°C

iGEM 2009 plates PC with internet connection :-) ddH20 0.5 ml sterile Eppendorf tubes

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