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CAROL
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WIKI CODING HERE
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CHINMOYEE
Wiki Wrap up / Team Meeting / Question Period
Scholarships , Posters , Presentations , timelines , future conferences and much more was discussed in the team meetings.
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EMILY
Wiki uploading madness, notebook updating and last team meeting
Today I spent the entire morning updating both the Wiki as well as my lab notebook. My lab Notebook is now up-to-date. Yay! And my section of the Wiki is almost completely updated. This afternoon we had an important team meeting to talk about or plans for the upcoming months in respect to the completion of our project.
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FAHD
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IMAN
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JAMIE
Analyzing AI-2 Isolation Data and Lab Cleanup
Lab cleanup! Lots of gruelling work but worth it. The lab looked really clean and spiffy after we were done with it.
Beyond that I also sat around and played with Excel to see what kind of graphs I could generate with my data and what use it would be. Data was re-arrayed with a macro donated by Margot from the Surette lab (MANY thanks with everything you have helped us with!!).
'Figure 1.'Luminescence readings from an autoinducer bioassay using cell-free supernatants from overnights of various bacterial strains with Vibrio harveyi MM32 as the reporter strain. MM32 is responsive (i.e. luminescent) to the AI-2 present in the supernatant of V. harveyi BB120, a wildtype strain, and BB152 (BB120 luxLM::Tn5)2, a strain deficient in AHL production. Salmonella typhimurium 14028 produces AI-2 but due to differences in boration1, specificity to the V. harveyi reporter strain, and therefore luminescence, decreases. S. typhimurium SS007 (14028 luxS::T-POP)3 is incapable of producing AI-2 and serves as a negative control. E. coli DH5α has been reported as lacking the ability to produce AI-24. The lack of a peak in luminescence for MM32 grown with DH5α supernatant validates using DH5α as the chassis for our system. Our signalling system in DH5α will be responsive solely to exogenously added AI-2 derived from BB152 as it only produces AI-2. Cell-free supernatants were made by centrifugation of overnight cultures and filtering it with a 0.2μm (indicated by an ‘F’). Controls indicate the importance of filtering the supernatant (data not shown). The graph indicates an average obtained from 3 trials.
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JEREMY
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KATIE
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KEVIN
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MANDY
Reformatting and adding to Updates
This week's blog posts have now been added to their respective update sections of the wiki, with some reformatting.
On a side note, the Hawaiian Bobtailed Squid has proved to be elusive and the search for one continues this week.
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PATRICK
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PRIMA
Wrap up
I spent all morning updating the wiki notebook, filling in details and filling in the details in my lab notebook. In the afternoon, we had a team meeting and question period.
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STEFAN
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VICKI
A picture upload frenzy
My notebook is written in paper and typed up in word documents. I copied/pasted those to the wiki and uploaded all of the pictures.
We also had a lab meeting where we discussed future directions for the project and partook in another Q&A session.
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