Make LB agar plates + antibiotic

Day 1

1. Make LB agar [[|How to Link]]
2. Send LB agar for autoclaving (before 12.30)

Day 2

1. Get LB agar from autoclave
2. Switch water bath to 50C
3. Microwave LB agar flask until agar dissolves
4. Put the LB agar flask into the water bath for 20-30 mins (this ensures the agar is dissolved properly)
5. Turn on the Bunsen burner. From now on work next to the flame for sterile technique.
6. 5 minutes before pipetting (see next step), take antibiotics out of the freezer and let them defrost
7. When antibiotics have defrosted, add 300μL of antibiotic to 300 mL of LB agar. Ratio of AB:LB is 1:1000