Team:Aberdeen Scotland/Registry
From 2009.igem.org
University of Aberdeen - Pico Plumber
BioBrick Experience
[http://partsregistry.org/Part:BBa_B0030 BBa_B0030]
Aberdeen_Scotland 2009 |
The miniprep, single and double digests all worked. However we did not use this part for further cloning. |
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
Aberdeen_Scotland 2009 |
Our miniprep, double digest and gel worked properly. We used this part in fusion PCR for building our biobrick . The fusion PCR, cloning and sequence were all correct. |
[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked as expected. |
[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
Aberdeen_Scotland 2009 |
Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality. |
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]
Aberdeen_Scotland 2009 |
Our miniprep, double and single digests worked as expected. We used this biobrick for building two of our parts ( and '). We experienced very satisfactory cloning with high efficiency of transformants. The sequencing was correct. We discovered that all the bacteria containing this biobrick were releasing fluorescence even though there was no promoter upstream. The level of fluorescence observed was dependent on the plasmid copy number. We noticed that colonies displayed a visible, yellow-green colour, therefore selection was very easy. |
[http://partsregistry.org/Part:BBa_I13600 BBa_I13600]
Aberdeen_Scotland 2009 |
The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. |
[http://partsregistry.org/Part:BBa_I13601 BBa_I13601]
Aberdeen_Scotland 2009 |
The gel analysis and the plasmid miniprep worked quite nicely as expected. In addition, the construct was further tested by adding IPTG which took off the lacRepressor of the LacO and hence enabling the CFP expression. |
[http://partsregistry.org/Part:BBa_I732100 BBa_I732100]
Aberdeen_Scotland 2009 |
The plasmid miniprep and the gel worked as expected. However, since we did not use this part for our cloning step we cannot comment on the sequencing or the other features of this part. |
[http://partsregistry.org/Part:BBa_I732820 BBa_I732820]
[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Aberdeen_Scotland 2009 |
Experimental Design Results Conclusion |
[http://partsregistry.org/Part:BBa_K081008 BBa_K081008]
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
Aberdeen_Scotland 2009 |
The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (54bp).But later it was confirmed following its usage by forming the part BBa_K182001 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked. |
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]
Aberdeen_Scotland 2009 |
The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked. |
[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]
Aberdeen_Scotland 2009 |
Our miniprep, digest and gel gave expected results. However we did not use this part for our cloning. |
[http://partsregistry.org/Part:BBa_S03518 BBa_S03518]
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked. Further testings were performed by the formation of the BBa_K182002, PCR analysis, sequencing and everything worked flawlessly, exceeding our expectations. |
[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and PstI) worked. Further testings were performed by the formation of the and , PCR analysis, sequencing and everything worked as expected. |
[http://partsregistry.org/Part:pSB1AT3 pSB1AT3]
Aberdeen_Scotland 2009 |
Plasmid rescued contained BBa_04450). The transformation, miniprep and the gel (undigested and digested with the EcoRI and PstI) worked. Further testings were performed through the PCR analysis and subsequent sequencing. |
[http://partsregistry.org/Part:pSB3C5 pSB3C5]
Aberdeen_Scotland 2009 |
The transformation was twice unsuccessful. Plasmid rescued contained BBa_J04450. |
[http://partsregistry.org/Part:pSB3T5 pSB3T5]
Aberdeen_Scotland 2009 |
Plasmid rescued contained BBa_J04450. Transformation, miniprep, double and single digests all worked properly. It was noticeable that colonies were a light pink colour. We used this part for further cloning. This plasmid gave us a very high efficiency of cloning. |
[http://partsregistry.org/Part:pSB4C5 pSB4C5]
Aberdeen_Scotland 2009 |
Mixed digestion results; digestion with EcoRI and PstI worked well in some cases and complete digestion was observed, however, in other cases incomplete digestion was observed. When pSB4C5-I52002 is transformed into XL1-blue cells, colonies are obtained. This is an unexpected results since this part contains the ccdB 'death' gene. |
[http://partsregistry.org/Part:pSB4T5 pSB4T5]
Aberdeen_Scotland 2009 |
The transformation was twice unsuccessful. Plasmid rescued contained BBa_I52001. |
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