Imperial College London/Notebook/1 October 2009
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Thursday the 1st of October
Testing team (update)
Harvard-GFP
- There is a difference in fluorescence between 28°C and 37°C after compensating for OD.
- Need to adjust the gain so that fluorescence doesn't saturate
- Runs: Wed 23rd September, 30th
OD calibration
- 17th of September
- 23rd of September
- 25th of September
- Preliminary results show that cell number varies with OD, repetition of the experiment will allow to fit a curve to the graph: cell number vs OD.
Secondary carbon sources
GFP pH
IPTG growth