Team:Imperial College London/Wetlab/Protocols/M1

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Protein Production (PP)

Section Assay Overview and Aims
PP1: IPTG Toxicity IPTG Toxicity To investigate the effect of our IPTG inducer on growth of our cultures.
  • Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined
  • Determine the effect of IPTG toxicity on growth w/o any protein production complications
PP2: Lac characterisation Lac characterisation

To investigate how RFP production induced by Lac promoter varies with IPTG

PP3: Cellulase Cellulase Aims
PP4: PAH PAH Aims

Mr. Gene   Geneart   Clontech   Giant Microbes

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