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ULB/12 July 2009
From 2009.igem.org
Contents |
July 12, 2009
Forward and reverse primers are designed and ordered.
Primers design for hfsG et hfsH genes
BBa_R0011 (promoter) :
PREFIX :
=> GAATTCATGAGATCT aattgtgagcggataaca
SUFFIX :
<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT
RBS BBa_B0034 :
PREFIX :
=> GAATTCATGAGATCT AAAGAG
SUFFIX :
<= ctcgagttaggatcc TTTCTC
hfsG gene :
PREFIX :
=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg
SUFFIX :
<= cagacggcctcgctgtaga
hfsH gene :
PREFIX :
=> atgccgatggagttcgagaag
SUFFIX :
<= ctcgagttaggatcc ttagagcccgatcc
RBS + RFP :
PREFIX :
=> GAATTCATGAGATCT aaagaggagaaatactag
SUFFIX :
<= ctcgagttaggatcc GCGATCTACACTAGCACT
BBa_B0015 terminator :
PREFIX :
=> GAATTCATGAGATCT ccaggcatcaaataaaac
SUFFIX :
<= ctcgagttaggatcc TATAAACGCAGAAAGGCC
Sequences
Promoter (55 bp)
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
RBS (12 pb)
aaagaggagaaa
RBS + RFP (681 bp)
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt gcttaataacgctgatagtgctagtgtagatcgc
Terminator (129 bp)
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
Plasmid (3165 bp)
ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct
