"
August/29 August 2009
From 2009.igem.org
1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location Approx. number of colonies
(12) 10
(13) 20
(14) 20
(15) 20
(16) over 100
(18) 3
(19) 20
(20) 0
(21) 0
1-6I over 100
Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.
2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.
3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector. Ligation check (by electrophoresis) should be conducted on these tomorrow.
