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User contributions
From 2009.igem.org
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- 08:34, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team (→Paolo Magni)
- 08:33, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 08:29, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 08:24, 23 June 2009 (diff | hist) File:FotoGruppo.jpg (uploaded a new version of "Image:FotoGruppo.jpg")
- 08:23, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 08:23, 23 June 2009 (diff | hist) File:FotoGruppo.jpg (uploaded a new version of "Image:FotoGruppo.jpg")
- 08:12, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 08:10, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 08:08, 23 June 2009 (diff | hist) N File:FotoGruppo.jpg
- 08:07, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 07:56, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 07:31, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 07:31, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 07:26, 23 June 2009 (diff | hist) Team:UNIPV-Pavia/Team
- 17:28, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/PCR (New page: <h1>PCR</h1> ''(estimated time: 3 hours and 30 min)'' <br> <br> '''Materials needed:''' *'''MgCl2''' *'''Buffer''' *'''dNTPs''' *'''ddH2O''' *'''Taq Polymerase''' *'''VF2 primer''' *'''VR...)
- 17:25, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/Ligation
- 17:24, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/Ligation
- 17:21, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/Ligation (New page: <h1>Ligation</h1> ''(estimated time: 20 min + 12-16 hours overnight incubation)'' <br> <br> '''Materials needed:''' *'''Roche T4 Ligase''' *'''10X Roche T4 Ligase Buffer''' *'''ddH2O''' <...)
- 17:20, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Protocols/Precipitation (New page: <h1>DNA precipitation with sodium acetate</h1> ''(estimated time: 1 hour)'' <br> <br> '''Materials needed:''' *'''sodium acetate''' *'''absolute ethanol''' *'''ethanol 70%''' *'''ddH2O'''...)
- 17:18, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/GelExtraction (New page: <h1>DNA gel extraction</h1> ''(Estimated time: 1 hour and 30 min)'' <br> <br> '''Materials needed:''' *'''Agarose gel with well-separated, sharp bands''' *'''Scalpel and tweezers to cut ge...)
- 17:17, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 17:16, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/Digestion (New page: <h1>BioBrick digestion with restriction enzymes</h1> ''(estimated time: 3 hours)'' <br> <br> '''Materials needed:''' *'''Roche restriction enzymes thawed on ice''' *'''Roche buffer H''' *...)
- 17:12, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 17:11, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction
- 17:09, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction (New page: <h1>Plasmid extraction</h1> ''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)'' <br> <br> '''Materials needed:''' *'''5 ml ...)
- 17:04, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 17:02, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/Resuspension (New page: {{UNIPV-Pavia/Protocols}} <br> == '''DNA resuspension from iGEM 2009 plates''' == ''(Estimated time: 30 min)'' <br> <br> '''Materials needed:''' *'''iGEM 2009 plates''' *'''PC with intern...)
- 16:58, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 16:51, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/Glycerol stock (→Gycerol stock)
- 16:51, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/Transformation (→Transformation)
- 16:48, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/XGal (New page: {{UNIPV-Pavia/Protocols}} <br> == '''X-Gal staining protocol for beta galactosidase (blue/white screening)''' == ''(Estimated time: 20 min)'' <br> <br> '''Materials needed:''' *'''40 mg/m...)
- 16:40, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/Transformation (New page: {{UNIPV-Pavia/Protocols}} <br> == '''Transformation''' == ''(estimated time: 3 hours and 30 min + 12-16 hours overnight incubation)'' <br> <br> '''Materials needed:''' *'''LB agar plates ...)
- 16:39, 22 June 2009 (diff | hist) N Team:UNIPV-Pavia/Methods Materials/LB (New page: {{UNIPV-Pavia/Protocols}} <br> == '''LB medium''' == ''(Estimated time: 10 min + 4 hours of autoclavation and cooling)'' <br> <br> '''Materials needed:''' *'''NaCl''' *'''BactoTryptone'''...)
- 16:33, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 16:20, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Methods Materials/Glycerol stock (→Gycerol stock)
- 16:18, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 16:16, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/StyleCss
- 16:14, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→Gallery)
- 16:13, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→Gallery)
- 16:12, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→Gallery)
- 16:11, 22 June 2009 (diff | hist) N File:Certosa-pavia.jpg
- 16:11, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→Gallery)
- 16:05, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 16:04, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 16:04, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 16:03, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 16:00, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→University)
- 15:55, 22 June 2009 (diff | hist) Template:UNIPV-Pavia/Protocols
- 15:53, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Pavia (→History)
- 15:51, 22 June 2009 (diff | hist) Team:UNIPV-Pavia/Notebook/Week3Jun (→June, 19th)
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