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- 12:27, 17 October 2009 (diff | hist) N Template:Team:KULeuven/1 September 2009/VanillinReceptor (New page: * There was a gel made to check if basicproduct W was correctly PCRed, and if the restriction digest of X,Y and K was good. * rpoA mutation on 202 was put on gel but there was no result * ...) (top)
- 12:21, 17 October 2009 (diff | hist) N Template:Team:KULeuven/31 August 2009/VanillinReceptor (New page: * TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector * The mutation of rpoA was again checked but gelele...)
- 12:15, 17 October 2009 (diff | hist) N Template:Team:KULeuven/28 August 2009/VanillinReceptor (New page: * new TOPO-cloning was done but with a change in protocol. Ligationtime of the fragement in the vector was changed from 5 minutes to overnight. *mutagenesis of rpoA on 202 was done but th...) (top)
- 12:10, 17 October 2009 (diff | hist) Template:Team:KULeuven/27 August 2009/VanillinReceptor (top)
- 12:09, 17 October 2009 (diff | hist) N Template:Team:KULeuven/27 August 2009/VanillinReceptor (New page: * Because of bad results yesterday the transformation of W was extra checked by performing a new PCR with different primers : sample A : M13forward - virAforward sample B : M13forward - v...)
- 12:01, 17 October 2009 (diff | hist) Template:Team:KULeuven/26 August 2009/VanillinReceptor (top)
- 11:58, 17 October 2009 (diff | hist) N Template:Team:KULeuven/26 August 2009/VanillinReceptor (New page: - PCR for the second mutation of R was done - Electroporation of B and G after the ligation yesterday - PCR was done for controle of W to check if virA was inserted into the cell. The pict...)
- 14:05, 10 September 2009 (diff | hist) N Template:Team:KULeuven/25 August 2009/VanillinReceptor (New page: * PCR mutagenesis from R on 427, PCR purification and gelelectrophoresis : no signal on the the picture probably because enting didn't showed enough result * Ligation van X and Y in K14 at...)
- 13:45, 10 September 2009 (diff | hist) N Template:Team:KULeuven/24 August 2009/VanillinReceptor (New page: * TOPO vector with insert W showed some colonies. PCR + electrophoresis were performed for control but a bad and unclear picture was made so the result was not that visible * B and G vecto...)
- 13:00, 26 August 2009 (diff | hist) N Template:Team:KULeuven/21 August 2009/VanillinReceptor (New page: * TOPO cloning of W looks goods with clear white colonies. 5 colonies were selected and cultured for testing) (top)
- 12:53, 26 August 2009 (diff | hist) Template:Team:KULeuven/20 August 2009/VanillinReceptor
- 12:48, 26 August 2009 (diff | hist) N Template:Team:KULeuven/20 August 2009/VanillinReceptor (New page: * Continued with ethanol precipitation, electroporation and outplating (of R mutation 202))
- 12:46, 26 August 2009 (diff | hist) Template:Team:KULeuven/18 August 2009/VanillinReceptor
- 12:44, 26 August 2009 (diff | hist) Template:Team:KULeuven/17 August 2009/VanillinReceptor (top)
- 12:42, 26 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 13:05, 25 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 13:01, 25 August 2009 (diff | hist) N Template:Team:KULeuven/19 August 2009/VanillinReceptor (New page: * Continued with ethanol precipitation for R. Electroporation and outplating were performed) (top)
- 12:59, 25 August 2009 (diff | hist) N Template:Team:KULeuven/18 August 2009/VanillinReceptor (New page: * PCR purification miniprep and nanodrop * start ethanol precipitatie (overnight ethanol + sodium acetate) * PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelel...)
- 12:54, 25 August 2009 (diff | hist) N Template:Team:KULeuven/17 August 2009/VanillinReceptor (New page: * A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out. * Fluid cul...)
- 12:47, 25 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 12:42, 25 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinReceptor
- 12:40, 25 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 12:39, 25 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 12:36, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 12:35, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 12:33, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 09:57, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 09:56, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 09:43, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 09:41, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 09:35, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 12:24, 12 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 11:57, 12 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 11:55, 12 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 09:56, 12 August 2009 (diff | hist) N Template:Team:KULeuven/12 August 2009/VanillinReceptor (New page: * B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commerci...)
- 09:46, 12 August 2009 (diff | hist) N Template:Team:KULeuven/11 August 2009/VanillinReceptor (New page: * We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampcillin. We isolated the...)
- 13:18, 10 August 2009 (diff | hist) N Template:Team:KULeuven/10 August 2009/VanillinReceptor (New page: * There was no result for our set up on Friday. We think there was a error with the protocol because with didn't shake before incubation. So we did a new transformation of every gene.)
- 12:24, 10 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 10:30, 7 August 2009 (diff | hist) N Template:Team:KULeuven/7 August 2009/VanillinReceptor (New page: * primers for mutagenesis have arrived * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used this frage...)
- 10:25, 7 August 2009 (diff | hist) N Template:Team:KULeuven/6 August 2009/VanillinReceptor (New page: * virA, virB, virG and rpoA were purified and nanodropped * all the genes were elongated with polyA-tales to make it possible to insert them in a TOPO-vector)
- 07:36, 6 August 2009 (diff | hist) Template:Team:KULeuven/5 August 2009/VanillinReceptor
- 07:35, 6 August 2009 (diff | hist) Template:Team:KULeuven/5 August 2009/VanillinReceptor
- 08:57, 5 August 2009 (diff | hist) N Template:Team:KULeuven/5 August 2009/VanillinReceptor (New page: * Gel electforesis was done to see if PCR has worked)
- 08:54, 5 August 2009 (diff | hist) Template:Team:KULeuven/4 August 2009/VanillinReceptor
- 08:27, 4 August 2009 (diff | hist) N Template:Team:KULeuven/4 August 2009/VanillinReceptor (New page: * The primers made for the directed mutagenesis on Friday were ordered)
- 08:23, 4 August 2009 (diff | hist) N Template:Team:KULeuven/3 August 2009/VanillinReceptor (New page: * Agrobacterium tumefaciens strain pTiA6NC/A1011 was plated out on LB-medium. * Primers for virA virG promotor region virB and RpoA have arrived)
- 08:26, 28 July 2009 (diff | hist) Team:KULeuven/About Us (→The Team)
- 07:56, 28 July 2009 (diff | hist) Team:KULeuven/Abstract (→concept)
- 07:52, 28 July 2009 (diff | hist) Team:KULeuven/Abstract (→concept)
- 19:03, 22 May 2009 (diff | hist) Team:KULeuven/Teamwork (→De iGEM 2009 KULeuven Managers)
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