Temporal Control

In our project there are 3 forms of temporal control that have been implemented.

• Chemical induction: Triggers the production of the polypeptide of interest using IPTG. Effectively 'kicks off' the system once the cell density is high enough.
• Autoinduction: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation.
• Thermoinduction: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
  
  
  

Explainations for temporal overview

Please click on the table for a clearer view.

Project Tour

For more details of the temporal control of the system, see the tabs below.

Temporal Control Contents