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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

Transformation

(estimated time: 3 hours and 30 min + 12-16 hours overnight incubation)

Put 1-2 µl of DNA resuspension or ligation into thaw TOP10 cells tube (every tube contains approximately 60 µl of competent cells). Incubate on ice for 30 min. Heat shock: 42°C for 1 min. Put transformed TOP10 tube on ice and then add 250 µl SOC medium. Incubate 1 hour at 37°C, 220 rpm. Plate 200 µl of the solution on a proper agar plate. Incubate overnight at 37°C.

LB agar plates with proper antibiotic Invitrogen TOP10 cells Resuspended DNA SOC medium

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