Team:Wisconsin-Madison/Restriction Digestion

Restriction Enzyme Mapping

Use smallest micro cuvettes and mix (total volume 18 μL):

• 1.8 μL Buffer (10x)
• 0.18 μL BSA +3 (100x)
• 12 μL MiliQ H20
• 3 μL DNA
• 1 μL Enzyme (100x)

Incubate in water bath at 37 C for 2 hours:

• 3.6 μL Dye (6x)