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Team:UNIPV-Pavia/Methods Materials/Glycerol stock
From 2009.igem.org
- LB medium
- DNA resuspension from iGEM 2009 plates
- Transformation
- X-Gal staining protocol for beta galactosidase (blue/white screening)
- Glycerol stocks
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- Electrophoresis
- DNA gel extraction
- DNA precipitation with sodium acetate
- Ligation
- PCR
- Sensing ethanol concentration: our do-it-yourself kit
Gycerol stock
(estimated time: 15 min + freezing time)
Materials needed:
- sample of overnight incubated bacteria
- 80% glycerol
- Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
- Leave at -20°C for one day
- Move to -80°C the day after
