Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

From 2009.igem.org

Revision as of 17:21, 22 June 2009 by Elisa df (Talk | contribs)

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)

Materials needed:

  • 5 ml or 9 ml (for higher yields) overnight mini-culture
  • QIAGEN QIAprep Spin Miniprep Kit


  • Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]