SLIC

Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method.

The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol (in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here].