Team:Stanford/NotebookPage/11 August 2009

Daily Work

Goals

• Revise diagram breakdowns for Jerome
• Revise vBOL symbols
• Either research Th-17 to NO pathway or work on wiki

Project / Accomplishments

• Looks like transformations worked!!!! Yay! (1.1b and 2.1a)
• Mini-preps of SoxR/S cultures and pcr on 1.1b and 2.1a
  • Need to sequence 1.2a
• Made revised constructions for Device 1
• New schematics for Diagrams
• vBOL symbols revised

Problems

• Simple schematic for Device 2....

Alternative Solutions

Events

• Meeting w/ Jerome for the presentation
  • Show Naive CD4+ cells leading to T-cells?
  • Testing line w/ filtering T-cells
  • Numbers
    • Rough percentage of amounts of T-cells (normal, non-recognizing, self-recognizing...)
  • Why don't some self-recognizing t-cells undergo apoptosis?
  • Circuit diagram
    • Make text bigger, animation,...
    • Take out IDO and dendritic cells?
  • Shorter descriptions
  • More visual support and animation
    • Specifically on slides describing why we need device 2
  • Device 2 parts breakdown- need separate promoter
  • Visual- pyramid w/ age group and
  • Immunology section- still too complicated
    • More pictures
    • Start w/ what the immune system "is" as a definition? Very general
  • Stay away from capital letters
  • Differentiate acquired and innate immune responses- mention vaccines!
    • Talk more about acquired w/ animation (fun can be good)
  • What is an antigen? What is an antibody?
  • Scheme is nice- could be more simple
  • Take away Tc cells
  • Selective filtering- make a factory line and assembly line
  • Define autoimmune diseases w/ respect to Self-recognizing t-cells
  • Start w/ epidemiology then immune response, then back to IBD w/ respect to the immune system
  • Mention system therapy vs. cure
  • Showing the cause of inflammation itself.... b-bells, t-cells, and plasma cells (igE?)-
    • Show transportation- lymph, vs blood, vs epithelial crossing
  • Where are Naïve CD4+ cells? Needs to be in the place of activity!!!
  • email Ariana or Professor Steinman

• Meeting w/ Christine for Stanford News at 2pm
  • Interviews, vidoes, and revised article
  • Looks good!

• FAB at 4:00
  • Trp looks better, but not perfect yet...
    • More modeling help needed?
  • IBD Overview
    • Be upfront of assumptions and parameters
      • We assume it is t-cell ratios for inflammation
  • Questions to answer
    • Where are Naive CD4+ cells?
    • What is the "cause" of IBD?
    • Why don't medicines target t-cells?
  • Also,
    • Future- how can we biosynthesize 5MT?

Comments

• Questions to be answered….
  • Genetic component of the disease?
  • Mouse model of IBD to test linear relationship of Th-17 and Treg cells
  • Suicide device?
  • Do people take RA for treatment?
    • The acid form of Vitamin A, all-trans retinoic acid or ATRA, has been made into Tretinoin, which
  • Do people take Il-6 for treatment?
  • Other uses?
  • Where are Naïve CD4+ cells? Needs to be in the place of activity!!!
• vBOL, are there names for the individual pictures